Coding
Part:BBa_K2817005:Design
Designed by: Zhaoyu Liu Group: iGEM18_NEU_China_A (2018-09-25)
YebF-Myrosinase (horseradish)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 493
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 722
Illegal BsaI site found at 1751
Design Notes
Combined with a vegetable diet, the sulforaphane produced by myrosinase can both alleviate inflammation in the intestine and prevent colorectal cancer induced by chronic inflammation. And we used YebF to help it arrive extracellular environment.
Source
It comes from cruciferous plant. We did a codon optimization for K12 strain and gave it to the company to synthesize it. YebF is a natural secretion tag in the E.coli.