Coding

Part:BBa_K2817005:Design

Designed by: Zhaoyu Liu   Group: iGEM18_NEU_China_A   (2018-09-25)


YebF-Myrosinase (horseradish)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 722
    Illegal BsaI site found at 1751


Design Notes

Combined with a vegetable diet, the sulforaphane produced by myrosinase can both alleviate inflammation in the intestine and prevent colorectal cancer induced by chronic inflammation. And we used YebF to help it arrive extracellular environment.


Source

It comes from cruciferous plant. We did a codon optimization for K12 strain and gave it to the company to synthesize it. YebF is a natural secretion tag in the E.coli.

References